Figure 3: Shiga toxin activity and subtyping. | Scientific Reports

Figure 3: Shiga toxin activity and subtyping.

From: Phylogenomic approaches to determine the zoonotic potential of Shiga toxin-producing Escherichia coli (STEC) isolated from Zambian dairy cattle

Figure 3

The top panel graph indicates the cytotoxic effect of selected STEC strain supernatants on Vero cells. Increased cell survival results in higher values. 0.2% Triton X-100 was used as a positive control; RPMI + LB and RPMI alone were used as negative controls. Values below the dashed line indicate a cytotoxic effect on the cells. 89% (16/18) of the STEC supernatants tested demonstrated a cytotoxic effect. Supernatants were prepared as described in Materials and Methods. Stx subtypes are shown in the lower panel along with ELISA results for detection of Stx. Isolates with both stx1a and stx2a are associated with higher toxicity. Sample number ZB-4-stx contains stx2a and exhibited cytotoxicity on Vero cells but was negative by ELISA.

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