Table 3 Primers list.
From: Histone methyltransferase Ash1L mediates activity-dependent repression of neurexin-1α
Purpose | Primer name | Sequence |
|---|---|---|
pnrxn1-Luc clone | pnrxn1-luc-F | ATGGGTACCCCTGGTGGGTTGCTCTTAAAGTC |
pnrxn1-luc-R | TAGCTCGAGCCCAAATTGAGCTCCTTTACCTG | |
EMSA Tag-DNA and paired-end tag primers | Tag A-nrxn1α promoter F | GTAAAACGACGGCCAGT CGCCCTTCTCTTTCAGAAGGACAG |
Tag A-nrxn1α promoter R | CAGGAAACAGCTATGAC GAGGAAGGGAAGAACATGGAACAG | |
Tag B-nrxn1α promoter F | CCTACACGACGCTCTTCCGATCT CGCCCTTCTCTTTCAGAAGGACAG | |
Tag B-nrxn1α promoter R | GCGGTTCAGCAGGAATGCCGAG GAGGAAGGGAAGAACATGGAACAG | |
Tag C-nrxn1α promoter F | GACGGCATTTTCGTTCTTATTAG GGGTGTGGGATCCAACAACCCAAC | |
Tag C-nrxn1α promoter R | GATCATAAAAGTATTAAAGTTC GGCATCCGAGCCCTTTCCCACCTC | |
Tag A-F | GTAAAACGACGGCCAGT | |
Tag A-R | CAGGAAACAGCTATGAC | |
Tag B-F | CCTACACGACGCTCTTCCGATCT | |
Tag B-R | GCGGTTCAGCAGGAATGCCGAG | |
Tag C-F | GACGGCATTTTCGTTCTTATTAG | |
Tag C-R | GATCATAAAAGTATTAAAGTTC | |
Pull down PCR | Nrxn1α-160-F | AAGGGGCTGCAGCTGCCAGCTGA |
Nrxn1α-160-R | CGTGACGCCGGGTGCTGTCCTTC | |
Nrxn1α-600-F | TCCTGCCTGCCCCTGCATTC | |
Nrxn1α-600-R | GGGCGGGGGTACCTGAGAAG | |
Nrxn1α-1.5K-F | GAAACTCACACCCACCCAATCCG | |
Nrxn1α-1.5K-R | TTTCCATCCAGAAAAACCAGAAC | |
Nrxn1α-2.0K-F | GGCCCTTGGTTCTGACCCTGTC | |
Nrxn1α-2.0K-R | TCCCCCAACATATCTATGGCCTG | |
Nrxn1α-3.8K-F | AAGAGGTCAGGGGCAATCCAAC | |
Nrxn1α-3.8K-R | TCACCAATGGCAAAAGTGAAGC | |
Nrxn2α promoter-F | GGGTGTGGGATCCAACAACCCAAC | |
Nrxn2α promoter-R | GGCATCCGAGCCCTTTCCCACCTC | |
Nrxn3α promoter-F | TTTCCAAAAATGTGCTGACATAGC | |
Nrxn3α promoter-R | TGGAGGTACTTCATACAGGGCAGG | |
β-actin promoter-F | CCACTGGGGCTCGCCCTATGCTTG | |
β-actin promoter-R | CCACTGGGGCTCGCCCTATGCTTG | |
NR2A promoter-F | GGGTTGCTGCGTTGCGTCTCGCTG | |
NR2A promoter-R | CTAGGGCACGCTTCTGCTGCGGTC | |
β-tubulin promoter F | CACACTACCGCAGTCTTCACGCTC | |
β-tubulin promoter R | GACGGTCTTGGGGGCTGGACTATG | |
Gapdh promoter-F | ATCCTGTAGGCCAGGTGATG | |
Gapdh promoter-R | AGGCTCAAGGGCTTTTAAGG | |
RT-PCR | Nrxn1α-RT-F: | CCAGCACAACCTGCCAAGAGGATTC |
Nrxn1α-RT-R | TGGGGCGGTCATTGGGAGGCCAC | |
Nrxn1β-RT-F | TCACCAGCATCCTTGCGAGGCGGAC | |
Nrxn1β-RT-R | TGGGGCGGTCATTGGGAGGCCAC | |
Nrxn2α-RT-F | CTACCTTCTGCTGGACATGGGCTCC | |
Nrxn2α-RT-R | GCGTGCTGCGGCTGTTCACA | |
Nrxn2β-RT-F | GTCTCGTCCAGCCTCAGCACCACC | |
Nrxn2β-RT-R | CGTGTACTGGGCCGGTCATTGGGA | |
Nrxn3α-RT-F | AGCGTCCCTGTGAAAATGGTG | |
Nrxn3α-RT-R | GATGGGGTTCTGGGACAGGTC | |
Nrxn3β-RT-F | GACTTGGCGACTTCCTCCAGC | |
Nrxn3β-RT-R | GCCAGTTGTCCACCTGAAGTG | |
NR1-RT-F | TCATCCTGCTGGTCAGCGATGAC | |
NR1-RT-R | AGAGCCGTCACATTCTTGGTTCCTG | |
NR2A-RT-F: | TGATGAACCGCACTGACCCTAAG | |
NR2A-RT-R | GGAAGAACGTGGATGTCGGATC | |
Ash1L-RT-F | AAGAGCCCAGTGAAAACATCAAC | |
Ash1L-RT-R | CCTCCTCCTGCTTCTGATGGA | |
Egr1-RT-F | GAGCGAACAACCCTATGAGC | |
Egr1-RT-R | AGCGGCCAGTATAGGTGATG | |
Gapdh-RT-F | CTCCCCCCCACCATCCGGGTTCCT | |
Gapdh-RT-R | CCTTGACTGTGCCGTTGAATTTGC | |
ChIP-PCR | Nrxn1 promoter-ChIP-F | TCCTGCCTGCCCCTGCATTC |
Nrxn1 promoter-ChIP-R | GGGCGGGGGTACCTGAGAAG | |
Nrxn1 5 UTR-ChIP-F | CAAGCTGAGAATGGCCTCAAAGCAC | |
Nrxn1 5 UTR-ChIP-R | CCCACTCCTGTGCCAAACCCAC | |
Nrxn1 3′UTR-ChIP-F | TGGTTCATCCCTAAGAAGTCAAC | |
Nrxn1 3′UTR-ChIP-R | TGGATAAATTGGCCTTGCAACAG | |
Gapdh promoter-ChIP-F | ATCCTGTAGGCCAGGTGATG | |
Gapdh promoter-ChIP-R | AGGCTCAAGGGCTTTTAAGG | |
Ash1L(+/−) genotyping | Ash1L-Wt-F | TTAGGCAAAAAGCCAGGGGCTATC |
Ash1L-Wt-R | CTCTTTATTCACTAGCCCTGGAAC |
