Figure 6: Interferon induces SAMHD1 expression in hepatic cell lines and in primary human hepatocytes.
From: Restrictive influence of SAMHD1 on Hepatitis B Virus life cycle
(a) HepG2 were cultured with 20 or 2000 U/mL, while (b) HepaRG cells were cultured with 2000 U/mL of interferon α2a (IFNα2a), interferon β (IFNβ) or interferon γ (IFNγ) for 20 hours. Cells were collected, lysed and probed for SAMHD1, STAT1 and GAPDH by Western blotting. (c) Primary human hepatocytes (PHH) from one donor were treated with 500 U/ml of IFNα2a or IFNγ for 3 or 24 hours. The relative level of SAMHD1 mRNA was determined by RT-qPCR. Fold-changes to untreated PHHs at the respective time points were calculated based on the median of three technical replicates. The mean ± SEM of the fold-changes of three technical replicates is represented. Statistical significance was determined using a one-way ANOVA with multiple comparisons according to Dunnett (*p < 0.05, **p < 0.005, n.s.: non-significant).
