Figure 8
From: Optical diffraction for measurements of nano-mechanical bending
Experimental estimation of dissociation constant of Vancomycin via saturation experiments in cantilevers is performed simultaneously with both techniques, reflective diffraction and OBDT.
(a) The dramatic bending observed on the functionalized cantilever is attributed to the surface pressure caused by the specific ligand-receptor binding of Vancomycin and a bio-mimetic bacterial cell wall target. In contrast, passivated cantilevers feature negligible bending. Equal equilibrium bending is estimated both by OBDT and diffraction detection techniques. The equilibrium deflection measured at different concentrations of Vancomycin are fitted by a Langmuir isotherm function. Identical values of the dissociation constant Kd are obtained using (b) OBDT and (c) our diffraction technique. Data points are mean values and error bars standard deviation, with n = 3.
