Figure 4
From: STMN1 Promotes Progesterone Production Via StAR Up-regulation in Mouse Granulosa Cells
Effect of STMN1 on ovarian GC steroidogenesis-related genes.
(a,c) Quantitative real-time RT-PCR analysis of ovarian GC steroidogenesis-related gene expression. GCs were transfected with Ad-control (siRNA-control) or Ad-Stmn1 (siRNA-Stmn1) and total mRNA was extracted and subjected to real-time PCR and data obtained from Ad-control (siRNA-control) transfected cells were set at 1.0. (b,d) Western blot analysis of StAR expression. After treatment for 48 hours, the GCs lysates were analyzed by immunoblotting with anti-StAR (ProteinTech Group) at 1:1000 dilution and anti-ACTB (ProteinTech Group) at 1:1000 dilution. ACTB was used as a loading control. *P < 0.05, **P < 0.01.
