Figure 4
From: MicroRNA-146a suppresses ROCK1 allowing hyperphosphorylation of tau in Alzheimer’s disease
Intra-hippocampal delivery of antagomir.
(A) The Y maze revealed that the percentage of spontaneous alternations was higher in 5xFAD mice treated with antagomir compared with Vehicle treated 5xFAD mice; and the Morris water maze showed shorter escape latency and higher percentage of time spent in the correct quadrant for 5xFAD mice treated with antagomir compared to Vehicle treated 5xFAD mice. (B) The relative expression of microRNA-146a (miR146a) was significantly inhibited in hippocampus, but not prefrontal cortex, after bilateral intra-hippocampal injections (n = 4). (C) The hippocampal protein levels of the related signaling pathway markers (ROCK1-pPTEN-pTau) were evaluated by Western blotting. A representative gel image is shown from four Control (wild-type), four Vehicle treated 5xFAD and four 5xFAD with antagomir mice. (D) The prefrontal protein levels of the related signaling pathways (ROCK1-pPTEN-pTau) were evaluated by Western blotting. A representative gel image is shown from four Control (wild-type), four Vehicle treated 5xFAD and four 5xFAD mice treated with antagomir mice. The data are shown as mean ± SE.
