Figure 3: Influence of a double salt bridge on the interaction between p63-DBD and various promotor sites.
From: Solution structure and binding specificity of the p63 DNA binding domain
p53-DBD forms an Arg-Glu’– Arg’-Glu double salt bridge between the two monomers in the dimer. In p63-DBD the glutamate is replaced by a leucine residue, thus reducing its affinity to all DNA promotor sites. The affinity is further reduced by mutation of the leucine to another arginine, but significantly enhanced if mutated to glutamate. Four different promotor sites were used: p53 specific con2 × 5, p21, Bax and p63 specific Jag-1.
