Figure 4: DNA binding of p63-DBD and p53-DBD.
From: Solution structure and binding specificity of the p63 DNA binding domain
(a) Titration of 2H,15N-labeled p63-DBD (blue) with 0.5 eq ds-con2 × 5 DNA (red) monitored with 2D-[15N, 1H]-TROSY experiments. (b) Chemical shift perturbations (CSPs) mapped onto a dimeric structure of p63-DBD bound to a DNA half site (pdb id: 3qym36). Regions in p63-DBD that are most affected are colored in red. (c) Sample spectra of 2H,15N-labeled p53-DBD upon titration with 0.5 eq ds-con2 × 5 DNA (blue vs. red contour lines). (d) CSPs mapped onto a crystal structure of dimeric p53-DBD bound to a decamer DNA site (pdb id: 2geq)42. In addition to the DNA-binding sites in p53-DBD, helix 1 shows strong CSP effects upon DNA binding caused by the formation of a double salt bridge with the second p53-DBD monomer.
