Figure 5: Interaction of p63-DBD with BclxL.
From: Solution structure and binding specificity of the p63 DNA binding domain
(a) Fluorescence polarization of fluorescein-labeled BclxLΔTM (without its insoluble transmembrane helix) upon titration of p63DBD yielding a KD of 38 ± 18 μM. Triplicate measurements were done to estimate standard error. (b) 2D-[15N; 1H] TROSY experiments of 200 μM p63-DBD alone (black) and in presence of a twofold excess of BclxLΔTM (red). (c) 1H, 15N averaged chemical shift perturbations (CSPs) of p63-DBD extracted from the spectra shown in (b). (d) Projection along the 1H dimension taken from 2D-TROSY spectra shown in (b) exhibit a pronounced decrease in signal intensity upon complex formation with BclxL (black: p63-DBD; red: in complex with BclxL). (e) CSPs mapped onto the solution structure of p63-DBD ranging from light grey (no effect) to red (>mean CSP + standard error).
