Table 1 Kinetic analysis of dissociation constant of all X-aptamers using ScrubberGen SPRi analysis software.

From: Nano-SPRi Aptasensor for the Detection of Progesterone in Buffer

X-Aptamer

KD (pM)

SD

DNA Sequence

1

999.74

0.069

TTTTT TT GATC CTTC--TCGTAYTGTCCACTGCGATCWCTT GATC TTTTT

2

49.51

0.099

TTTTT TT CTAG CTTCGTYTGTCTCAACGTGCCCTGCGWCGT GATC TTTTT

3

21.74

0.087

TTTTT TT CTAG AGATGTYTGTCTCAACGTGCCCTGCGWCGT GATC TTTTT

4

28.10

0.107

TTTTT TT CTAG AGAAGTYTGTCTTTCGCAYCAAGAGWTACG GATC TTTTT

5

25.58

0.081

TTTTT TT CTAG CTTCCGTGAAAYCAACGTGCCCTGGWWCGT CTAG TTTTT

6

53.03

0.061

TTTTT TT CTAG CTTCCGTGAAAYCAACGTGCCCTGGWTAGT CTAG TTTTT

7

140.37

0.064

TTTTT TT CTAG CTTCCGTGAAAYCAACGTGACCTGWWTAGT GATC TTTTT

8

40.79

0.076

TTTTT TT CTAG CTTCCGTGAAAYCAACGTGCCCTGGWAWCW GATC TTTTT

9

61.86

0.076

TTTTT TT CTAG CTTCCGTGAAAYCAACGTGCCCTGWWAWCG CTAG TTTTT

  1. The dissociation constant (KD value) reported was calculated for all 9 X-aptamers along with the standard deviation value (SD).
  2. W = dU with indole (tryptophan) attached to position 5.
  3. Y = dU with phenol (tyrosine) attached to position 5.
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