Figure 3 | Scientific Reports

Figure 3

From: Efficient suilysin-mediated invasion and apoptosis in porcine respiratory epithelial cells after streptococcal infection under air-liquid interface conditions

Figure 3

Adherence of S. suis to well-differentiated porcine bronchial epithelial cells.

PBEC were apically infected with approximately 1 × 107 CFU of S. suis wt, 10Δsly, 10ΔcpsΔsly, cW461F or cS148 respectively, After 4 h, cells were washed thoroughly to remove non-adherent bacteria. To determine adherence of streptococci to PBEC (A–E), immunostaining was applied to visualize cilia (red) and streptococci (green). Bars represent 50 μm. To Quantify the bacteria attached to PBEC (F) the areas containing green-fluorescent bacteria were determined from panels (A). Results are expressed as means ± SEM and significance, indicated by ***(P < 0.0001), was determined using one-way-ANOVA and Tukey multiple comparison test. Experiments were performed at least three times.

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