Figure 2 | Scientific Reports

Figure 2

From: Prolonged mitotic arrest induces a caspase-dependent DNA damage response at telomeres that determines cell survival

Figure 2

Sub-apoptotic caspase activation during mitotic arrest.

(A) Induction of apoptotic and sub-apoptotic caspase-3/7 activity. Asynchronous U2OS cells untreated (As) or pre-treated with actinomycin D for 24 h (ActD) were incubated with the fluorescent caspase-3/7 substrate DEVD-NucView 488 (NucView) and analysed by flow cytometry. Relative mean fluorescence intensities in sub-apoptotic and apoptotic cells ± SD from 3 independent experiments (right), one of which is shown (left). (B,C) Induction of sub-apoptotic caspase-3/7 activity by mitotic arrest. Asynchronous cells (As) or cells arrested in mitosis for 2 h with nocodazole (N2M) were incubated with (B) zVAD-fmk (zVAD) or (C) Navitoclax (Navi) or for a further 4 h with further addition of nocodazole in the case of N2M samples. Caspase-3/7 activity was assayed by flow cytometry using DEVD-NucView 488. Relative mean fluorescence intensities in non-apoptotic cells ± SD from 3 independent experiments are shown.

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