Figure 3

Agarose gel electrophoresis of microbial genomic DNA isolated from environmental and human samples.

Genomic DNA was electrophoresed on a 0.8% agarose gel, stained with ethidium bromide and photographed in a gel imaging system. (A) Genomic DNA isolated by THSTI method. Lane 1: Lambda genomic DNA digested with restriction endonuclease HindIII; lane 2: Genomic DNA isolated from Sewage water (SW), lane 3: Genomic DNA isolated from soil sample, lane 4: Genomic DNA isolated from stool, lane 5: Genomic DNA isolated from vaginal swab (VS), lane 6: Genomic DNA isolated from gastric tissue biopsy (GTB) sample. (B) Genomic DNA isolated from equal amount of samples using commercial kits or automated liquid handling system. Lane 1: Lambda genomic DNA digested with restriction endonuclease HindIII; Lane 2–3: Genomic DNA isolated from stool samples using commercial kit. Lane 4–5: Genomic DNA isolated from GTB samples using commercial kit. Lane 6–7: Genomic DNA isolated from stool DNA samples using automated liquid handling system. Lane 8–9: Genomic DNA isolated from VS samples using automated liquid handling system.