Figure 4

PCR amplification of 16S rRNA gene from community DNA isolated from environmental and human origin samples.

(A) Organization of conserved and variable regions of 16S rRNA gene. Small arrows indicate different primers used in this study to amplify partial or complete 16S rRNA gene. C denotes conserved while V indicates variable. (B) PCR amplification of complete or partial 16S rRNA gene using primers tagged with or without different barcode and adaptor sequences for 454 GS FLX+ pyrosequencer. Genomic DNA isolated both from environmental (SW, Soil) or human samples (Stool, VS, GTB) were used as template. Lane 1: 1-kb DNA ladder; Lane 2–6: complete 16S rRNA gene amplicons from SW, Soil, Stool, VS, GTB, respectively; Lane 7–11: V1-V5 region amplicons of 16S rRNA gene of SW, Soil, Stool, VS, GTB, respectively; Lane 12–16: V1-V3 region amplicons of 16S rRNA gene of SW, Soil, Stool, VS, GTB, respectively.