Table 1 Average concentration and total recovery of nucleic acids isolated from different environmental and human origin samples.
From: An Improved Method for High Quality Metagenomics DNA Extraction from Human and Environmental Samples
Sample | Method | Nucleic acid concn. (ng/μl) | Total recovery (ng) | 260/280 |
|---|---|---|---|---|
Stool | THSTI | 543.3 ± 187.26 (DNA) | 108660 ± 37520 (DNA) | 1.85 ± 0.06 |
Stool | Kit | 202.29 ± 105.63 (DNA) | 20229.23 ± 10563 (DNA) | 1.94 ± 0.23 |
Stool | ALHS | 113.38 ± 62.26 (DNA + RNA) | 11338.46 ± 6226 (DNA + RNA) | 1.67 ± 0.07 |
Vaginal Swab | THSTI | 104.77 ± 39.61 (DNA) | 20955.38 ± 7923.13 (DNA) | 1.69 ± 0.12 |
Vaginal Swab | Kit | 8.37 ± 5.66 (DNA) | 836.15 ± 566.7 (DNA) | 1.43 ± 0.58 |
Vaginal Swab | ALHS | 22.79 ± 9.5 (DNA + RNA) | 2279.23 ± 906.02 (DNA + RNA) | 2.47 ± 1.01 |
Soil | THSTI | 53.16 ± 36.77 (DNA) | 10633.84 ± 10317.18 (DNA) | 1.48 ± 0.041 |
Soil | Kit | 66.02 ± 70.13 (DNA) | 6602.30 ± 7014 (DNA) | 1.16 ± 0.05 |
Soil | ALHS | 93.91 ± 103.17 (DNA + RNA) | 9391.53 ± 7355.84 (DNA + RNA) | 1.44 ± 0.07 |
Sewage water | THSTI | 79.24 ± 80.71 (DNA) | 15849.23 ± 12190 (DNA) | 1.71 ± 0.041 |
Sewage water | Kit | 14.47 ± 5.72 (DNA) | 1447.69 ± 572 (DNA) | 1.68 ± 0.05 |
Sewage water | ALHS | 98.74 ± 60.95 (DNA + RNA) | 9874.61 ± 8071 (DNA + RNA) | 2.14 ± 0.07 |
Gastric Tissue Biopsy | THSTI | 53.9 (DNA) | 10780 (DNA) | 1.85 |
Gastric Tissue Biopsy | Kit | 126.5 (DNA+RNA) | 12650 (DNA+RNA) | 1.4 |
