Figure 7
From: Stemness and chemoresistance in epithelial ovarian carcinoma cells under shear stress
(a) The expression of phospho (p)-Akt, Akt, p-p70S6K and p70S6K in ovarian cancer spheroids cultured under static or 0.02 dyne/cm2 shear stress were analysed with western blot. The band intensity was determined by densitometry and data are shown as mean ± SEM. (b) Ovarian cancer spheroids were treated with or without 25 μM of LY294002 (c) or overexpressed with nonspecific (NS) miRNA or miR-199a-3p were subjected to 0.02 dyne/cm2 shear stress. The expression of Oct-4, ABCG2 and P-gp were analyzed by RT-PCR. The band intensity was determined by densitometry and data are shown as mean ± SEM. Significant differences (p < 0.05) between stationary and 0.02 dyne/cm2 shear stress are indicated with asterisk.
