Figure 4
From: Excess HB-EGF, which promotes VEGF signaling, leads to hydrocephalus
Expression of HB-EGF mRNA and the level of VEGF in the brain.
(a) A bar graph showing the level of VEGF mRNA in the forebrain including olfactory bulb and rostral subependyma of mice over-expressing HB-EGF (homozygotes) as compared to wild type. n = 3 in each group in this figure. (b) A bar graph with scattered data points displaying the level of CSF VEGF (pg/ml) in littermate controls (n = 3) and in the HB-EGF homozygous mice (n = 2). (c) Localization of the β-gal reporting human HB-EGF protein in the OB (coronal sections) at P21. N = 3 in each group for all the experiments in this figure. Single and double asterisks in a and b denote p < 0.01 and p < 0.05 by unpaired t test (two-tailed), respectively.
