Figure 1: Surface patterning and formation of lipid membranes with MBJs. | Scientific Reports

Figure 1: Surface patterning and formation of lipid membranes with MBJs.

From: Continuity of Monolayer-Bilayer Junctions for Localization of Lipid Raft Microdomains in Model Membranes

Figure 1

(A) The schematic diagram shows the cross-sectional view of a supported membrane across the MBJ in terms of the thickness of a lipid monolayer (Lt) and that of the S-PDMS layer (h). (B) A glass substrate in contact with the PDMS stamp with desired patterns for transfer printing at 200 °C for 3 min. (C) Removal of the PDMS stamp leaving behind grid patterns of the PDMS oligomers onto the glass substrate. (D,E) The exposure of the vesicles to the glass with the S-PDMS patterns (D) and the formation of a supported lipid membrane through spontaneous vesicular rupture across the MBJs on the S-PDMS patterned glass substrate (E). The phospholipids depicted are DOPC (represented by a white head group) and Texas Red-DHPE (red head group).

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