Figure 3: Effect of stamping temperature on lipid membranes formed on S-PDMS patterns.

Epifluorescence images of MBJs formed after S-PDMS patterning at increasing stamping temperatures. S-PDMS grids were patterned leaving 50 μm × 50 μm squares of bare glass (G) surrounded by S-PDMS (P). The MBJ features were formed by rupturing vesicles composed of DOPC with 1 mol % TR-DHPE. As a control experiment, a MBJ was formed on an S-OTS (O) grid stamped at room temperature (RT) (rightmost image).