Figure 5: Preferential localization of membrane components in lipid monolayers on S-PDMS patterns.

(A,B) Vesicles with various compositions were exposed to the glass support with the square patterns (50 μm × 50 μm) of S-PDMS. The MBJ features of the membranes composed of DOPC, NBD-CHOL, SPM, and GM1, where TR-DHPE was included (A) or excluded (B). (B–D), The MBJ features of the membranes composed of DOPC, TR-DHPE, and GM1, where NBD-SPM (C) or NBD-CHOL (D) was included. (E) The MBJ features of a membrane consisting of DOPC, TR-DHPE, NBD-PE, and GM1. The fluorescence intensities across the membranes along the dotted white lines (yellow curves; I1 for P region and I2 for G region) are shown in each micrograph. All scale bars are 100 μm.