Figure 6: Selective growth of raft domains in lipid monolayers and GM1/CTxB binding.

(A–D) Epifluorescence images and the corresponding intensity profiles across the S-OTS/glass (O/G) region (A,B) and the S-PDMS/glass (P/G) region (C,D) along the yellow dotted lines as function of time for a raft-forming lipid mixture (SPM/CHOL/DOPC /GM1/TR-DHPE = 33/33/32/1/1). (E–G), Schematic illustration of selective GM1 hetergeneity and the CTxB-GM1 binding process in the lipid raft microarray (E). Fluorescence images through Texas Red Filter (F) and Alexa Fluor 488 filters (G) after incubation of a solution of CTxB-488 for 1 h. All scale bars are 50 μm.