Figure 1: Generation and characterization of P. berghei ANKA uis3(–) parasites.
From: Protective efficacy and safety of liver stage attenuated malaria parasites
(A) Schematic of the uis3 replacement strategy using the 5′ and 3′UTRs of uis3 to integrate a resistance cassette by double crossover into the P. berghei strain ANKA genome. Locations of primers used for PCR in (B) are indicated. (B) Diagnostic PCR to investigate generation of uis3(–) parasites. WT: wild type, KO: uis3(–) parasite line. Numbers below the gel indicate expected amplicon sizes. (C) Liver stages in HepG2 cells formed by WT and uis3(–) sporozoites 65 hours post infection labeled with antibodies against EXP-1 and MSP1; Hoechst reveals host and parasite DNA. Images on the right show the merged images. Scale bar: 10 μm.
