Figure 2

GhLTPG1 is localized in plasma membrane and nuclear membrane and deletion of signal peptide or GPI domain or both results in uneven localization in plasma membrane.
(a) GhLTPG1 without signal peptide and GPI domain fused with eYFP. (b) GhLTPG1 without signal peptide fused with eYFP. (c) GhLTPG1 without GPI domain fused with. (d) GhLTPG1 fused with eYFP. (e) Tobacco epidermis cells transformed with CaMV35S::eYFP was used as the control. Upper column: fluorescent image in dark field. Middle column: bright field. Lower column: the overlay of fluorescent signals and bright field images. Scale bar: 50 μm.