Figure 5 | Scientific Reports

Figure 5

From: GhLTPG1, a cotton GPI-anchored lipid transfer protein, regulates the transport of phosphatidylinositol monophosphates and cotton fiber elongation

Figure 5

GhLTPG1 colocalizes with the biomarkers of PtdIns3P (2xFYVEmHrs-mCherry) and PtdIns4P (PHhOSBP1-eGFP) in cell membrane of tobacco epidermis cells.

(a) GhLTPG1 colocalizes with PtdIns3P in cell membrane of tobacco epidermis cells. Subcellular localization of GhLTPG1-eYFP (left) and PtdIns3P (2xFYVEmHrs-mCherry) in tobacco epidermis cells (middle) was observed. The overlay of GhLTPG1 and PtdIns3P is shown (right). Bars = 50 μm. (b) GhLTPG1 colocalizes with PtdIns4P in cell membrane of tobacco epidermis cells. Subcellular localization of GhLTPG1-mCherry (left) and PtdIns4P (PHhOSBP1-eGFP) in tobacco epidermis cells (middle) was observed. The overlay of GhLTPG1 and PtdIns4P marker is shown (right). Bars = 50 μm. Stars and arrows indicate the fluorescently labeled GhLTPG1 formed into vesicle and moved along the filament, respectively.

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