Figure 3
From: Anomalous uptake and circulatory characteristics of the plant-based small RNA MIR2911
Superior Stability of MIR2911 compared to other plant microRNAs in in vitro digestion.
The stability of MIR2911, MIR168 and C7 were assayed in an in vitro digestion system that includes a gastric digestion phase and a intestinal digestion phase. Three different samples were digested: (a) cabbage lysate containing a measured amount of 10 pmoles of plant-derived MIR2911 and spiked-in 10 pmoles each of synthetic 2′ O-methylated MIR168 and C7; (b) 10 pmoles each of synthetic 2′ O-methylated MIR2911, MIR168 and C7; (c) 10 pmoles each of synthetic MIR2911, MIR168 and C7 without 2′ O-methylations. Aliquots of digestion reaction were removed at various time points for analysis: 30 min, 60 min in gastric phase, 5 min, 30 min and 75 min in intestinal phase. Levels of surviving miRNAs were measured by qRT-PCR. The surviving percentages were calculated by comparing the levels of miRNAs at each time point to their starting levels before digestion.
