Figure 7
From: Anomalous uptake and circulatory characteristics of the plant-based small RNA MIR2911
Circulatory forms of MIR2911 in mice fed plant-containing diets.
(a) Sera were obtained from mice fed either chamomile, honeysuckle, or chow diet. Exosomes were isolated from these mouse sera via ultracentrifugation protocol (UC) or PEG precipitation protocol (PEG). Levels of miR-16 and MIR2911 were quantified by qRT-PCR in the exosome pellets, or the supernatant fractions. (b) Size exclusion chromatography analysis of Let-7a, miR-16 and MIR2911 levels in control (untreated) serum samples from mice fed vegetable diets. (c) Time course analysis of miR-16 and MIR2911 levels in proteinase K-treated or control (untreated) serum samples from mice fed vegetable diets. (d) Size exclusion chromatography analysis of Let-7a, miR-16 and MIR2911 levels in proteinase K-treated serum samples from mice fed vegetable diets. Note: in Panel (b) and (d), the scale for Y axis for the 3 miRNA targets are set independently. E.g. for miR-16, the peak1 in panel (b) (arrow) represents similar concentration as the peak1 in panel (d) (arrow).
