Figure 2
From: Characterisation of assembly and ubiquitylation by the RBCC motif of Trim5α
The Bbox and elements of the coiled-coil fold as a single domain.
Design and characterization of RhT5α 88–261dCC EK/RD. (a) Structure of the Bbox-coiled-coil region from Trim5α (PDB id:4TN317) Predicted structure of RhT5 88–261dCC with designed linker shown as dashed line is shown inset. Residues from monomer A are coloured blue and monomer B are coloured orange. Zinc atoms are shown as grey spheres. (b) SEC-MALLS analysis. Chromatogram from the differential refractive index detector are shown at two protein concentrations. Mw measured across the peak corresponding to a monomeric species is shown as open circles. (c) Sedimentation velocity AUC c(S) analysis at three protein concentrations. (d) SAXS curves between 60–480 μM with inset guinier analysis. (e) Comparison of SAXS scattering at 480 μM (6 mg/ml) to predicted model shown in (a). (f) ab initio bead model from SAXS generated in GASBOR compared to the predicted model shown in (a).
