Figure 3
From: Characterisation of assembly and ubiquitylation by the RBCC motif of Trim5α
Trim5α coiled-coil contributes to assembly (a) SEC-MALLS analysis of RhT5 88–261dCC shows an increase in elution position and Mw with increasing concentration. (b) SV-AUC c(S) analysis of RhT5 88–261dCC over a concentration range of 5–160 μM. Normalised c(S) profiles are shown. (c) (upper) Multispeed SE-AUC of RhT5 88–261dCC. Data were collected at three starting concentrations and fit taking into account time and radially independent noise. A single starting concentration (20 μM) is shown. (lower) Residuals of the fit to a monomer-trimer equilibrium with dissociation constant of 0.98 nM2. (d) SAXS curves between 60–480 μM with guinier curves inset. (e) ab initio models of the RhT5 88–261dCC trimer were produced using DAMMIF36. Selected raw models are shown in orange, consensus averaged model generated in DAMAVER is shown in green.
