Figure 2
From: Ring-Shaped Microlanes and Chemical Barriers as a Platform for Probing Single-Cell Migration
Bimodal analysis of cell tracks.
(a) Typical trajectory of an MDA-MB-436 cell confined within a ring-shaped pattern. The radial cell position, r, is plotted against the tangential position, dtang = R · φ, calculated as the product of the mean lane radius, R = 50 μm and the cell’s angular position φ. The track exhibits periods of directionally persistent as well as periods of localized, erratic motion. The perimeter of the ring is 314 μm. The shown cell performs more than one full revolution. (b) Distance covered along the tangential direction of the ring-shaped lane (top), tangential component of cell velocity (middle) and classification into run and rest states (bottom) for the track shown in (a). The bimodal analysis reveals the run (blue) and rest (red) periods in the cell track. (c) Expanded view highlighting the different character of the trajectory in run (blue) and rest (red) periods. (d) Distribution of the mean velocity in the tangential direction, 〈v〉state, within each run and rest period for more than 200 MDA-MB-436 cells. (e) Log-linear plot of the survival function, S(t) = P(T > t), of the persistence times of the run (blue) and rest (red) states. For both states, S(t) is fitted by an exponential distribution (dashed lines).
