Table 1 List of target genes, guide RNAs (gRNA), target sequences and PAM sequences used in this study.
From: Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9
target gene | gRNA | target sequence | PAM | reference |
|---|---|---|---|---|
NtFT4 | gFT4_Sa | GCCCAAGCAACCCTAACCTGA | GGGAGT | This study |
gFT4_Sp | CCCAAGCAACCCTAACCTGA | GGG | This study | |
NtPDS | gPDS_Sa | TTGCGATGCCTAACAAGCCAG | GGGART | This study |
gPDS_Sp | TGCGATGCCTAACAAGCCAG | GGG | This study | |
OsDL1 | gDL-1_Sa | GTCTTTTGGGTAGCTGCAGGT | TGGAGT | This study |
gDL-1_Sp | TCTTTTGGGTAGCTGCAGGT | TGG | Mikami et al.25 | |
gDL-2_Sa | GACTGACCTGAAACGGGCCCA | AGGGGT | This study | |
gDL-2_Sp | ACTGACCTGAAACGGGCCCA | AGG | Mikami et al.25 | |
OsCYP72A33 | gCYP72A33_Sa | CGTACGAGGTGGATTGCTGGC | CGGAGT | This study |
OsCYP72A32 | gCYP72A33_Sa | CGTACGAGGTGGATTGCTGGC | CGGAGC | This study |
OsVIP1 | gVIP1_Sa | TAAGAGGAGCGGGTCGATGGA | TGGGGT | This study |
OsVIP1-like | gVIP1_Sa | TAAGAGGAGCGGGTCGATGGA | TGGGGA | This study |
