Table 3 Mutation rate of T0 regenerated tobacco plants with mismatched bases in the target sequence.
From: Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9
target gene | gRNA | No. of mismatched bases | Cas9 | No. of examined plants | No. of plants with mutations | Mutation rate (%) |
|---|---|---|---|---|---|---|
NtFT1 | gNtFT4_Sa | 2 | SaCas9 | 32 | 0 | 0 |
NtFT2 | gNtFT4_Sa | 4 | SaCas9 | 32 | 0 | 0 |
