Table 4 Mutation rate of transformed rice callus in target sequences with non-canonical PAM for SaCas9.

From: Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9

target gene

gRNA

target sequence

PAM

Mutation rate (%)

OsDL

gDL-3_Sa

GCCAGCTCCTGAATGTTCATG

AGGAAG

0

OsALS

gALS-2_Sa

GGACCTTGCACTGACTGCAGG

AGGAAC

0

OsLIG6

gLIG6_Sa

ACGGAGAGCGATTTGGAGTCC

GGGGAG

0

OsPDS

gPDS-1_Sa

CCGTCCAACCCATTCCTCTGC

AGGAGC

8.3

OsPOLQ

gPOLQ-1_Sa

TGTATATAGCACAGAGCTCAG

GGGGAA

15.7

  1. Differences from the canonical PAM “5-NNGRRT-3” for SaCas9 are underlined.
  2. Os ALS: Oryza sativa acetolactate synthase, OsLIG6: Oryza sativa DNA ligase 6, OsPDS: Oryza sativa phytoene desaturase, OsPOLQ: Oryza sativa DNA polymerase Q.
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