Figure 3: Lineage tracing of RBP-Jκ deficient Ngn3 progenitors during development.
From: Early pancreatic islet fate and maturation is controlled through RBP-Jκ
(a-b’) Combined immunostaining for Insulin and Glucagon (red), YFP (green), on controls (a) and RBP-Jκ (b-b’) pancreatic anlages at E15.5. The lower panels presents the same images with an overlay for the colocalization of Insulin + Glucagon in white. (d) Immunostaining for CP-A (red) and YFP (green) on E15.5 pancreatic rudiments of control (c) and RBP-Jκ mice with close-ups of the highlighted areas underneath (d). Immunostaining on E18.5 pancreatic sections for Synaptophysin (red) and YFP (green) in control (e) and RBP-Jκ mice (f–h). All nuclei were counterstained with DAPI (blue). Scale bars = 50 μm.
