Figure 7: Lineage-tracing of RBP-Jκ deficient β-cells.
From: Early pancreatic islet fate and maturation is controlled through RBP-Jκ
Immunostaining for Insulin (blue), Amylase (red) and YFP (green) on RIP-Cre; YFP control (a) and RIP-Cre; RBP-Jκf/f; YFP (in b) mice. Panels a’,b’ present confocal imaging for Insulin (red) and YFP (green). Panels a”,b” display an overlay of the colocalization of the two channels in white. Panels (c–f) show confocal images for immunostaining for Insulin in blue and YFP in green with either Synaptophysin (c), Glucagon (d), Somatostatin (e) or Pancreatic Polypeptide (f) in red, with the corresponding overlay representation for the red and green channels in white in the corresponding panels below (c’–f’). Scale bars = 50 μm.
