Figure 5: Native polyacrylamide gel electrophoresis (PAGE) (7%, 1 × TAEMg) analysis of the detection systems.
From: A Versatile Multiple Target Detection System Based on DNA Nano-assembled Linear FRET Arrays
(a) The representative two probe-composed system (P1-P2) for one target (T1) detection. Lane 1: gel confirmation of the quantitative hybridization of probe P1 and P2, Lane 2: PAGE analysis of the P1-P2 system in the presence of target stand T1; (b) The representative three probe-composed system (P1-P2-P3) for two target (T1 or/and T2) detection. Lane 1: gel confirmation of the quantitative hybridization of probe P1, P2 and P3, Lane 2 (or Lane 3): PAGE analysis of the P1-P2-P3 system in the presence of target stand T1 (or T2), Lane 4: PAGE analysis of the P1-P2-P3 system in the presence of target stands T1 and T2; (c) The representative four probe-composed system (P1-P2-P3-P4) for three target (T1 or/and T2 or/and T3) detection. Lane 1: gel confirmation of the quantitative hybridization of probe P1, P2, P3 and P4, Lane 2: PAGE analysis of the P1-P2-P3-P4 system in the presence of target stand T1, Lane 3: PAGE analysis of the P1-P2-P3-P4 system in the presence of target stand T1 and T2, Lane 4: PAGE analysis of the P1-P2-P3-P4 system in the presence of target stand T1, T2 and T3.
