Figure 1: Identification of proteins affected by the lesR overexpression through 2-D gel proteome analysis of Lysobacter enzymogenes OH11.

(A) Monitoring and comparison of the growth curve between the lesR overexpression and control strains in 1/10 TSB broth. The time point used for cell collection was set at OD₆₀₀1.0 (indicated by the dotted line). Data are from three independent biological experiments. Each experiment involved three replicates for each strain. (B) Overexpression of lesR almost impaired the HSAF production in strain OH11. The HSAF production (indicated by the red arrow) from the collected cells, as noted in part (A), was extracted and determined by HPLC. (C) Functional classification of the identified 33 proteins affected by the lesR overexpression. The detailed information of each gene described in this figure was provided in the Table 1. OH11(lesR), the lesR overexpression strain; OH11(pBBR), the wild-type strain containing an empty expressing vector.