Table 2 Bacterial strains and plasmids used in this study.
From: A TonB-dependent receptor regulates antifungal HSAF biosynthesis in Lysobacter
Strains and plasmids | Properties or characteristics a | Source |
|---|---|---|
Lysobacter enzymogenes | ||
 OH11 | The wild-type strain, KmR | CGMCC No. 1978 |
 OH11(pBBR) | OH11 harboring plasmid pBBR1-MCS5, GmR, KmR | |
 OH11(lesR) | OH11 harboring plasmid pBBR-lesR, GmR, KmR | |
 ΔTBDR1 | TBDR1 in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR2 | TBDR2 in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR4 | TBDR4 in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR7 | TBDR7 in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR8 | TBDR8 in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR9 | TBDR9 in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR1&7 | A double in-frame mutation in TBDR1 and TBDR, KmR | This study |
 ΔtonB | tonB in-frame deletion mutant of strain OH11, KmR | This study |
 ΔTBDR7(TBDR7) | ΔTBDR7 harboring plasmid pBBR-TBDR7, GmR, KmR | This study |
 ΔTBDR7(pBBR) | ΔTBDR7 harboring plasmid pBBR-MCS5, GmR, KmR | This study |
 ΔTBDR7(TBDR7-V74A) | ΔTBDR7 harboring plasmid pBBR-TBDR7, GmR, KmR | This study |
Escherichia coli | ||
 DH5a | F−, φ80dlacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk−,mk+), phoA, supE44, λ−, thi-1, gyrA96 | Lab collection |
Plasmids | ||
 pEX18GM | Suicide vector with a sacB gene, GmR | |
 pBBR1-MCS5 | Broad-host-range vector with a Plac promoter, GmR | |
 pBBR-lesR | pBBR1-MCS5 cloned with a 1525-bp fragment containing the coding region of lesR and its predicted promoter region | |
 pEX18-TBDR1 | KmR, pEX18GM with two TBDR1 flanking fragments | This study |
 pEX18-TBDR2 | KmR, pEX18GMwith two TBDR2 flanking fragments | This study |
 pEX18-TBDR4 | KmR, pEX18GM with two TBDR4 flanking fragments | This study |
 pEX18-TBDR7 | KmR, pEX18GM with two TBDR7 flanking fragments | This study |
 pEX18-TBDR8 | KmR, pEX18GM with twoTBDR8 flanking fragments | This study |
 pEX18-TBDR9 | KmR, pEX18GM with twoTBDR9 flanking fragments | This study |
 pEX18-tonB | KmR, pEX18GM with twoTonB flanking fragments | This study |
 pBBR-TBDR7 | pBBR1-MCS5 cloned with a 3266-bp fragment containing the coding region of TBDR7 and its predicted promoter | This study |
 pBBR-TBDR7(V74A) | pBBR-TBDR7, where the amino acid V74 within the TonB-box region was substituted by A74 | This study |
