Figure 2: Pcgf6 Regulates the Core Circuitry and Maintains Pluripotency in ESCs.
From: Polycomb Group Protein Pcgf6 Acts as a Master Regulator to Maintain Embryonic Stem Cell Identity
(A) Micrographs showing abnormal EB morphology of differentiating Pcgf6-mESCs. CCE ESCs were stably transfected with Pcgf6 or EGFP (control), and in vitro differentiation was assessed by monitoring EB formation over 15 days. Images were taken using phase contrast microscopy from day 12 cultures of control cells and 4 individual clones overexpressing Pcgf6. Scale bar = 100 μm. (B,C) Expression of ESC-specific (B) and differentiation marker (C) genes during in vitro differentiation of EGFP- or Pcgf6-overexpressing CCE ESCs. mRNA levels were measured on days 0, 3, 6, 9, 12, and 15 and are presented as the ratio of mRNA levels relative to the highest expression level during differentiation. Four biological replicates are shown in four different color lines and control (EGFP-overexpressing CCE) is in black dotted line.
