Figure 6: Reversible inhibition of liver NAT activity by apocynin.

Apocynin inhibition of liver NAT activity appears to be reversible upon dilution. Liver lysates were incubated with varying amounts of apocynin (0–1 mM) in the assay for 30 min. The samples were subsequently diluted 1000 times, and evaluation of residue liver NAT activity was conducted using AcCoA and pANS as substrates, as described in methods. Assays were conducted in triplicates.