Figure 2 | Scientific Reports

Figure 2

From: The novel compound Sul-121 inhibits airway inflammation and hyperresponsiveness in experimental models of chronic obstructive pulmonary disease

Figure 2

Effects of Sul-121 on LPS-induced neutrophilia in BALF, blood and airway tissue.

Guinea pigs were treated for 3 min by inhalation of vehicle or Sul-121 (3 or 30 mM nebulizer concentration) 30 min before intranasal instillation of 300 μl saline or LPS (5 mg/ml) at 0 h. After animal termination at 25 h, BAL were performed and blood and lungs were collected for further analysis. Neutrophils were stained with TNAP (red staining, arrows) in transverse frozen cross-sections of the lung. Representative images are shown (A), magnification: 10X, inset, 20X). Neutrophils in cartilaginous (B) and non-cartilaginous airways (C) on sections were quantified in the adventitia and sub-mucosa and expressed as the number of positively stained cells per mm basement membrane length. Neutrophil numbers were determined in BALF (D) and blood (E). N = 4–6. (B,C,D) **p < 0.01, ***p < 0.001; two way ANOVA with bonferroni post-tests.

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