Figure 3 | Scientific Reports

Figure 3

From: The novel compound Sul-121 inhibits airway inflammation and hyperresponsiveness in experimental models of chronic obstructive pulmonary disease

Figure 3

Effects of Sul-121 on blood H2S level and lung CBS expression.

Guinea pigs were treated for 3 min by inhalation of vehicle or 30 mM Sul-121 at 30 min before intranasal instillation of 300 μl saline or LPS (5 mg/ml) at 0 h. After animal termination at 25 h, blood/serum was collected and H2S levels were measured (A). Correlations between serum H2S levels and neutrophil numbers in non-cartilaginous (B) were determined. Western analysis of CBS protein expression was performed in lung homogenates. Cropped images are shown. Full-length blots are presented in Supplementary Fig. 2. Gels have been run under the same experimental condition (C). CBS expression (yellow brown staining, arrows) was determined using immunohistochemistry in transverse frozen cross-sections of the lung. Representative images are shown (D). N = 4–6. A: n.s: not significant versus saline vehicle.

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