Figure 4 | Scientific Reports

Figure 4

From: The novel compound Sul-121 inhibits airway inflammation and hyperresponsiveness in experimental models of chronic obstructive pulmonary disease

Figure 4

Effects of Sul-121 on lung Nrf2 expression and MDA level in lung homogenates.

Guinea pigs were treated for 3 min by inhalation of vehicle or 30 mM Sul-121 at 30 min before intranasal instillation of 300 μl saline or LPS (5 mg/ml) at 0 h followed by animal termination on 25 hours. Western analysis of Nrf2 protein expression was performed in lung homogenates. Cropped images are shown. Full-length blots are presented in Supplementary Fig. 3. Gels have been run under the same experimental condition (A). Nrf2 expression (yellow brown staining, arrows) was determined using immunohistochemistry in transverse frozen cross-sections of the lung. Representative images are shown (B). MDA levels were measured in lung homogenates (C). Correlation between lung MDA concentration and total airway neutrophil numbers was performed (D). N = 4–6. (C) *p < 0.05, **p < 0.01; two way ANOVA with bonferroni post-tests.

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