Figure 5 | Scientific Reports

Figure 5

From: The novel compound Sul-121 inhibits airway inflammation and hyperresponsiveness in experimental models of chronic obstructive pulmonary disease

Figure 5

Effects of Sul-121 on IL-8 release and p65 nuclear translocation in ASM cells.

Immortalized human ASM cells were incubated with Sul-121 (10–300 μM) in the absence of presence of 15% CSE for 24 h. Fenoterol (1 μM) served as positive control. IL-8 concentrations in supernatants were measured by ELISA (A). Cells were trypsinized for trypan blue cell counting to determine cell viability (B). For immunofluorescence of p65 (C), hTERT cells were incubated with 300 μM Sul-121 in the absence and or presence of 15% CSE for 2 hours. Representative images are shown. Images were quantified by Image J 1.48v (D). (A,B) N = 7–24; ***p < 0.001 compared to CSE vehicle; two way ANOVA with Bonferroni post-tests; ##p < 0.01, ###p < 0.00, compared to corresponding basal IL-8 release, two way ANOVA with Bonferroni post-tests. D: N = 5; **p < 0.01, **p < 0.001; two way ANOVA with Bonferroni post-tests.

Back to article page