Figure 6
Effects of Sul-121 on ROS production.
Under cell-free condition, carboxy-H2DCF-DA (0.1 μM) was incubated for 40 min with 300 μM Sul-121 in the absence and presence of H2O2 (2 mM) or CSE (15%). ROS was measured by the intensity of DCF emission (A). Alternatively, DHE (5 μM) was incubated for 1 hour with Sul-121 (1–100 μM) in the absence and presence of H2O2 (5 μM). ROS was measured by the intensity of DHE emission (B). Immortalized human ASM cells were incubated with 300 μM Sul-121 in the absence and presence of PMA (0.1 μM) for 2 hours. 10 mM NAC served as positive control. After removal of the supernatant, cells were incubated with carboxy-H2DCFDA (0.1 μM) for 1 h. ROS was measured by the intensity of DCF emission (C). (A) N = 12; **p < 0.01, ***p < 0.001; two way ANOVA with Bonferroni post-tests. (B) N = 3, ***p < 0.001, compared to Basal; one way ANOVA with Bonferroni post-tests. ##p < 0.01, ###p < 0.001, compared to H2O2 treatment; one way ANOVA with Bonferroni post-tests. (C) N = 4; *p < 0.05, **p < 0.01, ***p < 0.001; two way ANOVA with Bonferroni post-tests.
