Figure 7
From: Mechanical perturbations trigger endothelial nitric oxide synthase activity in human red blood cells
Inhibitors of NOS activation decrease NO production by static and vortexed RBCs.
RBCs were pre-incubated with diamide or specific inhibitors of band3, Src kinase, or PIK3. Washed RBCs were incubated with DAF-FM (5 μM) for 30 minutes and subjected to static or vortex mode, followed by measurement of NO production at 495/515 nm. Vortexed RBCs produced significantly more NO than static RBCs in the absence of inhibitors. Increasing concentrations of diamide (a), band3 inhibitor (b), src-kinase inhibitor (c) and PIK3 inhibitor (d); caused significant and dose-dependent decrease in NO production by vortexed RBCs compared with static RBCs (n = 3; *p ≤ 0.014).
