Figure 5: TJ-M2010-2 interferes with DC maturation and decreases T cell proliferation.

(a) Bone marrow cells from BALB/c mice were cultured with GM-CSF and IL-4 to induce the production of BMDCs. Seven days later, DCs were incubated with TJ-M2010-2 for one hour and then stimulated with LPS for 48 h. CD80, CD86 and MHC-II levels were measured by FCM. TJ-M2010-2 inhibited CD80, CD86 and MHC-II levels dose-dependently (one of three independent experiments). (b) Quantitative analysis of the results above. (*P < 0.01 versus Control; ^※p < 0.05 versus LPS; ^#p < 0.01 versus LPS; † p < 0.01 versus 10 μM). Results are expressed as mean ± s.d. Blank: Unstained DCs; Control: DCs stained with CD80, MHC-II, CD86 and CD11c antibodies in the absence of intervention. (c) Lymphocytes as responder cells were obtained from C57BL/6 mice and stained with CFSE. BMDCs as stimulator cells were derived from BALB/c mice and treated with LPS with or without TJ-M2010-2. The lymphocytes were co-cultured with BMDCs at a 10:1 ratio for four days, and the proliferation of CD4⁺ and CD8⁺ T cells was measured by FCM. The proliferation index (PI) was regarded as the parameter (one of three independent experiments). PI is the total number of divisions divided by the number of cells that went into division. (d) Quantitative analysis of the results from MLR. (^*p < 0.01 versus Control; ^※p < 0.05 versus LPS; ^#p < 0.01 versus LPS; ^†p < 0.01 versus 10 μM). Results are expressed as mean ± s.d. Control: CD4⁺/CD8⁺ T-cells stained with CFSE and co-cultured with DCs in the absence of intervention.