Figure 5 | Scientific Reports

Figure 5

From: Medial plantar nerve ligation as a novel model of neuropathic pain in mice: pharmacological and molecular characterization

Figure 5

ATF-3 immunoreactivity in the spinal cord after MPNL or CCI.

At 3 or 7 days after MPNL or CCI surgery, spinal cords were harvested and the expression of ATF-3 was evaluated by immunofluorescence. ATF-3 was detected exclusively in the nuclei of the ventral horn of the spinal cord of CCI mice. Representative images are shown in (A–F). (G) ATF-3 + neurons visualized (indicated by arrows) in the dorsal horn of the spinal cord are also shown at a higher magnification. (H) As a control, the primary antibody was omitted from the reaction. Scale bar 250 μ m. (I) The number of ATF-3 + neurons are also shown. The data are presented as the mean ± S.E.M (n = 4 per group). ***P < 0.001 compared with naive mice. #P < 0.001 compared with MPNL mice.

Back to article page