Figure 6
Expression and anti-microbial activity of CvT-def 1 and 3.
(A) Expression levels of CvT-defs from 6–48 h post-challenge with B. subtilis, E. coli, C. albicans, or PBS (control) as measured by qPCR. Each time point and treatment was replicated 3 times with error bars indicating SE. For each time point, different letters indicate significantly different means (p < 0.05). (B) Outcomes of zone inhibition and kinetic growth assays using CvT-def 1 and 3 against B. subtilis and E. coli. For each treatment the left image shows a culture plate and inhibition zones which are identified 1–5, + and −. Concentrations for CvT-def 1 are 4.55 μM (1), 2.28 μM (2) 1.14 μM (3), 0.57 μM (4) and 0.24 μM (5). Concentrations for CvT-def 3 are 8.77 μM (1), 4.39 μM (2), 2.19 μM (3), 1.1 μM (4), 0.55 μM (5). Ampicillin at 50 μg/ml is the positive control (+) while 0.01% acetic acid is the negative control (−). The right image shows graphs for the growth kinetic experiments using 2× and 4× concentrations of the MICs determined for CvT-def 1 and 3. Ampicillin at 50 μg/ml or 0.01% acetic acid served as controls. Growth kinetics were monitored at OD600 over 360 min with each treatment replicated 3 times. Error bars for each treatment and time point indicate SE.
