Figure 3
From: The NKD1/Rac1 feedback loop regulates the invasion and migration ability of hepatocarcinoma cells
NKD1 interacts with Rac1 and promotes its degradation via ubiquitin-proteasome pathway.
(A) NKD1 slightly co-localized with Rac1 in the cytoplasm which is displayed as yellow areas. (B) Co-immunoprecipitation experiments indicated that NKD1 interacts with Rac1 in 293T and HepG2 cells. (C) MG132 treatment prevented NKD1-mediated Rac1 degradation. (D) NKD1 significantly shortened the half-life of Rac1. (E) The addition of NKD1 to Rac1-transfected HCC cells increased Rac1 poly-ubiquitination.
