Table 1 Primers sequences.
From: The NKD1/Rac1 feedback loop regulates the invasion and migration ability of hepatocarcinoma cells
RT-PCR | Primer name | F:5′-3′ | R:5′-3′ |
|---|---|---|---|
| Â | NKD1 | CAGCGGAGATGAGAAGAAGATG | CAAAGTCATACAGGGTGAAGGT |
|  | β-actin | ATAGCACAGCCTGGATAGCAACGTAC | CACCTTCTACAATGAGCTGCGTGTG |
| Â | Rac1 | GTTATGGTAGATGGAAAACCGG | TTGCTTTTCCCTTGTGAGTC |
shRNA | NKD1-1 | ccggtAGGAGAAACCACTACTTAGTTCAAGAGACTAAGTAGTGGTTTCTCCTTTTTTTACGCGTg | aattcACGCGTAAAAAAAGGAGAAACCACTACTTAGTCTCTTGAACTAAGTAGTGGTTTCTCCTa |
| Â | NKD1-2 | ccggtGCCATGAACATCACCACCATTTCAAGAGAATGGTGGTGATGTTCATGGTTTTTTACGCGTg | aattcACGCGTAAAAAACCATGAACATCACCACCATTCTCTTGAAATGGTGGTGATGTTCATGGCa |
| Â | NKD1-3 | ccggtGCCAAGAAGCAGCTGAAGTTTTCAAGAGAAACTTCAGCTGCTTCTTGGTTTTTTACGCGTg | aattcACGCGTAAAAAACCAAGAAGCAGCTGAAGTTTCTCTTGAAAACTTCAGCTGCTTCTTGGCa |
Pbobi-cmv | NKD1 | CCGCTCGAGATGGATTACAAGGATGACGACGATAAGGGGAAACTTCACTCCAAGCC | CGGAATTCCTATGTCTGGTAGAAGTGGT |
| Â | HA-UB | GCTCTAGAATGTACCCATACGACGTCCCAGACTACGCTCAGATCTTCGTGAAAACCCTT | CCGCTCGAGTTAACAGCCACCCCTCAGGCGCAGGACCA |
| Â | MYC-Rac1 | GCTCTAGAATGTACCCATACGACGTCCCAGACTACGCTCAGGCCATCAAGTGTGTGGTGGT | CCGCTCGAGTTACAACAGCAGGCATTTTCT |
| Â | EZH2 | TGCTCTAGAATGGGCCAGACTGGGAAGAAATC | CCGCTCGAGTCAAGGGATTTCCATTTCT |
PGL3 | NKD1 | CCGCTCGAGTACTGAATGAAGAATTGTAAC | CATGCCATGGGCTGGGGCTGCGGCGGCC |
